TECHNIQUES OF PURIFICATION of ANTIGEN O ISOLATES of Escherichia coli AND IN VIVO ANALYSIS OF ANTIGEN IMMUNOGENICITY IN POULTRY

Lipopolysaccharide (LPS) is a major surface component of most Gram-negative bacteria and recognized by immune cells as pathogen-associated molecules. Antibodies against O antigens can provide protection infection vaccines have been developed conjugating to carrier proteins enhance immunogenicity. This study aims produce antigen from Escherichia coli analyze the in vivo test results poultry using ELISA technique. The methods used this were E. Bacterial Culture, Antigen Purification, SDS PAGE, Inoculation on Experimental Animals, Rapid Plate Agglutination Test, ELISA. Based research conducted, it be concluded that injected into chickens induce formation specific antibodies blood. Specific formed detected rapid plate agglutination